Petaka Options

PetakaG3 Basic Concept

PetakaG3 basic concepts

Dimensions:

Inches: 5.03 x 3.38 x 0.21

mm: 127.8 x 85.7 x 5.45

Maximum media volume: 25 mL

Maximum cell culture surface: 150 cm²

Characterization:

The cell culture device PetakaG3 is an innovative bioreactor designed for mammalian cell culture in physiologic tissue conditions.

The distinctive feature of PetakaG3 is that being a disposable device incorporates an automatic gas diffusion control system (AGD-CS) that works interactively with the cells growing and living inside, WITHOUT HYPOXIA CHAMBERS, GLOVE BOXES OR THREE-GAS INCUBATORS.

For cells cultured in PetakaG3 and incubated in regular 21% oxygen atmosphere the AGD-CS provides:

1) Media dissolved oxygen concentration within the limits of the physiologic oxygen tensions in physiologic tissues (from 75 mmHg down to 15 mmHg) monitorable with a dissolved oxygen fluorescent sensor dot. Because this advanced trait PetakaG3 is the safest, smallest, unique portable disposable hypoxia chamber, also available with integrated DO sensor.

2) Media dissolved CO2 concentration, enough to maintain a physiologic pH without external extra-supply of carbon dioxide gas.

3) Controlled media evaporation even at 2% to 10% atmospheric Relative Humidity (RH).

PetakaG3 is available in several options for culturing all kinds of cells from vertebrate and invertebrate organisms, especially suited for stem cells, primary cultures and tumor cells. PetakaG3 LOT & PetakaG3 HOT with hydrophilic surface developed for anchored dependent cells growth and PetakaG3 FLAT with a hydrophobic surface, not permissive of cell adhesion, for non adherent cells growth and 3D cellular clusters and aggregates. PetakaG3-Matrix is available carrying different surface molecular matrices, such as Collagen, Vitronectin, Matrigel, etc. and also structural matrices (nano-fibers mesh), or combinations of structural and molecular.

4) PetakaG3 allows cell cultures of two different cell types in the same device. Both cell layers are separated by 3mm of media, which is appropriate to study intercellular interactions through diffusible factors and exosome exchange. PetakaG3 is ideal for transfection of both cell layers with two different gene constructs in the same device with negligible cross transfection

PetakaG3™ LOT (Low Oxygen Transfer)

PetakaG3 LOT (Low Oxygen Transfer)

Distinctive: Blue port

The cell culture device PetakaG3 is an innovative bioreactor designed for mammalian anchorage dependent cell culture in Oxygen physiologic tissue conditions. The distinctive feature of PetakaG3 is that being a disposable device incorporates an automatic gas diffusion control system (AGD-CS) that works interactively with the cells growing and living inside, WITHOUT HYPOXIA CAMBERS, GLOVE BOXES OR THREE-GAS INCUBATORS

For cells cultured in PetakaG3 and incubated in regular 21% oxygen atmosphere the AGD-CS provides:

Average DO = 1 mg/L (0.5 to 1.1 mg/L depending on cell type), equivalent to average 22.7 mmHg (Torr) in tissues or equivalent to average 3.11 %O2 in hypoxia chambers.

2) Media dissolved CO2 concentration, enough to maintain a physiologic pH without external extra-supply of carbon dioxide gas.3) Controlled media evaporation even at 2% to 10% atmospheric Relative Humidity (RH).PetakaG3 allows cultures of two different cell types in the same device. Both cell layers are separated by 3mm of media, which is appropriate to study intercellular interactions through diffusible factors and exosome exchange. PetakaG3 is ideal for transfection of both cell layers with two different gene constructs in the same device with negligible cross transfection

Global specifications of PetakaG3 LOT:

a.- It acts as an independent hypoxia chamber between 1% and 21% Oxygen. Medium Dissolved Oxygen auto-controlled (O2PP between 2 and 50 mmHg)

b.- No additional CO₂ required for any type of media, although the PetakaG3™device can also be used in CO₂ incubators

c.- Humidity autocontrolled, no added humidity required (not influenced by environment)

d.- The most secure cell culture device against contamination (no caps, no openings)

e.- Designed to transport live cells without requiring freezing and dry ice

f.- Optimizes media and growth factor supplies

g.- Can provide up to 15 times more cell culture surface area per incubator capacity

h.- Both surfaces may be viewed using any type of light microscope

i.- In vitro cell pause or dormancy is easily accomplished

j.- Allows storage of pre-filled units that are ready to host cells

h.- Easy to handle. It operates in any position. Completely safe & versatile

j.- Each Petaka is individually bar coded for easy trackinL.

PetakaG3 DO-Sensor

PetakaG3 DO sensor

Distinctive: Sensor inside

The cell culture device Petaka DO-Sensor, with O2 sensor, Integrates all advantages of the Petaka LOT, HOT and Petaka-ET with the precision of the advance Dissolved Oxygen Monitoring technology of Ocean Optics, Inc.
PetakaG3 DO-Sensor has a fluorescent Oxygen mini-sensor of approximately 4 mm in diameter built inside (RedEye® Oxygen Patches with the FOXY coating). The sensor has an insignificant thickness which does not affect cell growth and expansion.
This sensor allows either continuous or intermittent readings of the DO levels in the cell culture media across unlimited time span, in a non-intrusive manner.A microspectro fluorimeter, Oxymeter NeoFox®, and a specific friendly and intuitive software ( NeoFox® Phase Measurement System) emits light pulse flashes to the sensor and retrieves fluorescent responses from it. The processor of this system calculates the precise amount of DO through the intensity of the sensor emitted light and the exact temperature of the media. Read scientific foundation of the system. DO is expressed in ppm, mmHg partial pressure, molar concentration and as a percentage value in atmosphere displayed as real time values and graphics.DO amounts and related values used in the calculations (such as temperature and fluorescence emitted per pulse) can be recorded in EXCEL type tables exportable into any type of statistical and graphic software The Petaka G3 DO-Sense

oxygen-sensitive fluorescent probe makes possible non invasive monitoring of cellular oxygen consumption of the ceell culture, avoiding the necessity of sampling. This external sensor-reading procedure allows rapid and specific detection of cellular oxygen consumption providing a direct sensitive means of assessing the impact of a given experimental condition on cellular metabolism.
Areas of application include:
Drug discovery, development and testing
Screening for cell toxicity compounds
Optimization of cell culture conditions

PetakaG3™ ET™ (Easy Transfer)

NEW SURFERS

PetakaG3 ET (Easy Transfer)

Distinctive: Stirrers inside

The cell culture device PetakaG3™ ET™ bioreactor is a PetakaG3™ that includes inside the cell culture chamber a set of free steel doped micro-bars (stirrer bars) that can be moved by magnetic fields.

The Petaka™ ET bioreactor is used in conjunction with either an automatic magnetic random scanner (Cell Flipper) that drags the stir bars across the internal surface of Petaka or with a manual magnetic tablet (Cell lifter) on which the user drags the Petaka to detach the cells.

The turbulences created by the stir bar motion over the cells are strong enough to induce cell enzyme free cell detachment without the need for chemicals. Depending on cell type the automatic scanning is fast for cell harvesting and preserves a high yield of unharmed detached cells that can be directly transferred to new bioreactors without washing.

The manual Cell Lifter is used for detaching fragile cells carefully; the operator controls the speed and the specific areas of the cell monolayer to be detached.

Petaka G3 ET is ideal for enzyme free detachment for any kind of cell line, and extremely well suited for Stem cell cultures, also allows for safe and frequent cell culture samplings during growth, and in vitro evaluation of wound healing, or Gap Closure Migration Assays.

PetakaG3™ HOT (High Oxygen Transfer)

ptak g3 hot cell culture celartia

PetqkaG3 HOT (High Oxygen Transfer)

Distinctive: Orange port

The cell culture device Petaka G3 with orange color port.

PetakaG3™ HOT bioreactor is manufactured from a special co-polymer which has a particularly high diffusion rate of oxygen and carbon dioxide through the walls of the device.

Average DO = 3 mg/L (2.8 to 3.2 mg/L depending on cell type), equivalent to 66.5 mmHg in tissues or equivalent to 9.33 %O2 in hypoxia chambers.

Because of its high diffusion coefficients requires to be incubated in 5% CO2 with saturated relative humidity to avoid dehydration.

PetakaG3™ HOT has a gas self regulating system that maintains the level of dissolved oxygen in the cell culture media like in all Petaka G3, but the minimal level that can reach is higher, for a given cell type and cell density. Example: in the same cell culture conditions (cell type, cell density and media type) when Petaka G3 balances the oxygen exchange at 1ppm, Petaka G3 HOT will hold it at 3ppm, maintaining the median level of dissolved oxygen in the highest limit of the physiologic oxygen tension of the tissues, equivalent to 10% concentration of oxygen in the open atmosphere.

PetakaG3™ HOT bioreactor is particularly suited for culturing cells with a high aerobic metabolism, for example primary liver cell cultures, hematopoietic bone marrow cells, well differentiate keratinocytes, hybridomas, Pancreatic islets, etc.

Cells cultured for a long time in traditional flasks and dishes, exposed to 21% oxygen of open atmosphere, are metabolically adapted to such environment, far from the in vivo reality and behavior.

PetakaG3™ FLAT (Suspended Cell Culture)

PetakaG3 FLAT (For Low ATachmet)

Distinctive: White port

This bioreactor has all of the same size and structure characteristics as the PetakaG3™ either LOT or HOT bioreactor, except that the interior surfaces are not treated to promote cell adherence to its walls.This special bioreactor is produced with a flat polymer with a very low surface tension.The dissolved Oxygen control is identical to all Petaka G3, so it maintains a physiologic Oxygen tension adjusted to the cell type needs in vivo.

Applications:

Suspended cell cultures such as; Blood cell preparation, Leukemia Cells, Hybridomas Cells, Lymphocyte culture and activation for kariotyping.

Special cells necessary for the bio-synthesis of specific products for pharmaceutical applications.

Cultures of cells with self agregation such as spheroids, 3D cultures, pseudo-tissues, etc.

PetakaG3™ MATRIX (Specific Molecular Matrix)

The cell culture device Petaka G3 MATRIX, could be LOT or HOT

Distinctive: Green port

The PetakaG3™ MATRIX bioreactor has all of the same size and structure characteristics as the PetakaG3™ bioreactor.

PetakaG3™ MATRIX is Coated with Specific Molecular Matrix. These are several types of Petaka internally coated with specific molecular matrix incluiding: Collagen, Fibronectin, Poly-L-lysine, Matrigel, Gelatin, Laminin.

This product is usually best suitable for growing different types of stem cells.

Because of the specialty matrices involved, these bioreactors are only available on a made-to-order basis.