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Dr. Barbera and Dr. Forteza regularly contribute topical Biology articles and news associated with cellular physiology, cellular pathology, cell culture, materials, equipment, methodologies and research. You are encouraged to subscribe via the RSS links to support email alerts to your inbox whenever new content is posted here.
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Physiologically Relevant Cell Culture, Is it true?

spheroids hypoxia evaluation celartia
excess 0xygen flask G
  Physiologically Relevant Cell Culture   As early as 1969 Shaw and Pace reported that: “Cells tolerate a low O 2  exposure (5%) for at least 20 days while continuous exposure to high O 2  atmosphere results in degeneration and death after 7–10 days” (1). These were one of the first experiments showing that cells in culture survive better in...
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Petaka G3 uses for hybridoma culture and shipment

A) Culturing Hybridomas 1A.- Seed 1M or 2M Hybridoma Cells in Petaka G3-FL AT(HOT or LOT), depending on cell line oxygen requirement and fill it with the selected hybridoma special media   2A.- After cell injection, mount the Petakas in a “stack” with stands, and live them in the incubator in horizontal position for 12 hours . Face Up  ...
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