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Dr. Barbera and Dr. Forteza regularly contribute topical Biology articles and news associated with cellular physiology, cellular pathology, cell culture, materials, equipment, methodologies and research. You are encouraged to subscribe via the RSS links to support email alerts to your inbox whenever new content is posted here.

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spheroids hypoxia evaluation celartia
  Physiologically Relevant Cell Culture   As early as 1969 Shaw and Pace reported that: “Cells tolerate a low O2 exposure (5%) for at least 20 days while continuous exposure to high O2 atmosphere results in degeneration and death after 7–10 days” (1). These were one of the first experiments showing that cells in culture survive better in hypoxia although no explanation was...
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A) Culturing Hybridomas 1A.- Seed 1M or 2M Hybridoma Cells in Petaka G3-FLAT(HOT or LOT), depending on cell line oxygen requirement and fill it with the selected hybridoma special media   2A.- After cell injection, mount the Petakas in a “stack” with stands, and live them in the incubator in horizontal position for 12 hours . Face Up   3A.-...
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